AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Bedienungsanleitung

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Inhaltsverzeichnis

Seite 1 - Getting Started Guide

Before You BeginDesigning Primers and Probes for Quantification AssaysDesigning Primers and Probes for Allelic Discrimination AssaysOrdering Primers a

Seite 2

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-01.fmChapter 1 Before You BeginIntroductionPrimer Express Software 3.0 Getting Started Guide 3Notes1Prim

Seite 3 - Chapter 4

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-01.fmChapter 1 Before You BeginInstalling the Software4 Primer Express Software 3.0 Getting Started Guid

Seite 4 - Contents

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-01.fmChapter 1 Before You BeginInstalling the SoftwarePrimer Express Software 3.0 Getting Started Guide

Seite 5

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-01.fmChapter 1 Before You BeginBasics6 Primer Express Software 3.0 Getting Started GuideNotesBasicsStart

Seite 6 - How to Use This Guide

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmPrimer Express Software 3.0 Getting Started Guide 7NotesChapter 22Designing Primers and Probes forQ

Seite 7 - How to Obtain Support

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysOverview8 Primer Express Software 3

Seite 8 - Before You Begin

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysAutomatically Designing Primers and

Seite 9

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysAutomatically Designing Primers and

Seite 10 - Introduction

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysAutomatically Designing Primers and

Seite 11 - Installing the Software

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysAutomatically Designing Primers and

Seite 12 - Chapter 1 Before You Begin

© Copyright 2004, Applied Biosystems. All rights reserved.For Research Use Only. Not for use in diagnostic procedures.Information in this document is

Seite 13

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysManually Designing Primers and Prob

Seite 14 - Quantification Assays

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysManually Designing Primers and Prob

Seite 15 - Overview

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysManually Designing Primers and Prob

Seite 16 - To load a sequence file:

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysManually Designing Primers and Prob

Seite 17 - Finding Primers

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysManually Designing Primers and Prob

Seite 18

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysManually Designing Primers and Prob

Seite 19 - Saving the

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysAutomatically Designing Primers for

Seite 20

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysAutomatically Designing Primers for

Seite 21

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysAutomatically Designing Primers for

Seite 22 - If primers were not found:

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysAutomatically Designing Primers for

Seite 23

DRAFTDecember 16, 2004 3:56 pm, GSG_Workflow.fmBefore You BeginChapter 1Designing Primersand Probes forQuantificationAssaysChapter 2Designing Primersa

Seite 24 - To design the Reverse Primer:

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysManually Designing Primers for SYBR

Seite 25

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysManually Designing Primers for SYBR

Seite 26 - Green Dye

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysManually Designing Primers for SYBR

Seite 27 - Green Dye Assays

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-02.fmChapter 2 Designing Primers and Probes for Quantification AssaysManually Designing Primers for SYBR

Seite 28

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmPrimer Express Software 3.0 Getting Started Guide 27NotesChapter 33Designing Primers and Probes for

Seite 29

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmChapter 3 Designing Primers and Probes for Allelic Discrimination AssaysOverview28 Primer Express S

Seite 30

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmChapter 3 Designing Primers and Probes for Allelic Discrimination AssaysAutomatically Designing Pri

Seite 31

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmChapter 3 Designing Primers and Probes for Allelic Discrimination AssaysAutomatically Designing Pri

Seite 32

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmChapter 3 Designing Primers and Probes for Allelic Discrimination AssaysAutomatically Designing Pri

Seite 33

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmChapter 3 Designing Primers and Probes for Allelic Discrimination AssaysAutomatically Designing Pri

Seite 34 - Allelic Discrimination Assays

DRAFTDecember 16, 2004 3:54 pm, GSG_4tabsTOC.fmContentsPrimer Express Software Version 3.0 Getting Started Guide vPreface viiChapter 1 Before You Begi

Seite 35

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmChapter 3 Designing Primers and Probes for Allelic Discrimination AssaysAutomatically Designing Pri

Seite 36

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmChapter 3 Designing Primers and Probes for Allelic Discrimination AssaysManually Designing Primers

Seite 37 - Assigning a SNP

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmChapter 3 Designing Primers and Probes for Allelic Discrimination AssaysManually Designing Primers

Seite 38

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmChapter 3 Designing Primers and Probes for Allelic Discrimination AssaysManually Designing Primers

Seite 39

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmChapter 3 Designing Primers and Probes for Allelic Discrimination AssaysManually Designing Primers

Seite 40

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-03.fmChapter 3 Designing Primers and Probes for Allelic Discrimination AssaysManually Designing Primers

Seite 41

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-04.fmPrimer Express Software 3.0 Getting Started Guide 39NotesChapter 44Ordering Primers and ProbesOrder

Seite 42

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-04.fmChapter 4 Ordering Primers and ProbesOverview40 Primer Express Software 3.0 Getting Started GuideNo

Seite 43

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-04.fmChapter 4 Ordering Primers and ProbesOrdering Primers and ProbesPrimer Express Software 3.0 Getting

Seite 44 - To design the Forward Primer:

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-04.fmChapter 4 Ordering Primers and ProbesOrdering Primers and Probes42 Primer Express Software 3.0 Gett

Seite 45

vi Primer Express Software Version 3.0 Getting Started GuideDRAFTDecember 16, 2004 3:54 pm, GSG_4tabsTOC.fm

Seite 46 - Ordering Primers and Probes

Primer Express Software 3.0 Getting Started Guide 43IndexAadd DNA file 9, 19, 29allele 2, 34, 36, 37allelic discriminationcreating the document 29alle

Seite 47

44 Primer Express Software 3.0 Getting Started GuideIndexSNP Target 30SNP target 30SNP Target Tool 31Starting and Exiting Primer Express Software 6SYB

Seite 48

Worldwide Sales and SupportApplied Biosystems vast distribution and service network, composed of highly trained support and applications personnel, re

Seite 49

DRAFTDecember 16, 2004 3:54 pm, GSG_Preface.fmPrimer Express Version 3.0 Getting Started Guide viiPrefaceHow to Use This GuidePurpose of ThisGuideThe

Seite 50

DRAFTDecember 16, 2004 3:54 pm, GSG_Preface.fmPrefaceHow to Obtain More Informationviii Primer Express Version 3.0 Getting Started GuideExamples of th

Seite 51

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-01.fmPrimer Express Software 3.0 Getting Started Guide 1Notes1Chapter 1Before You BeginBefore You BeginI

Seite 52 - Part Number 4362460 Rev. B

DRAFTDecember 16, 2004 3:54 pm, GSG_Chapter_4-01.fmChapter 1 Before You BeginIntroduction2 Primer Express Software 3.0 Getting Started GuideNotesIntro

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