AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Bedienungsanleitung PDF herunterladen (Seite 40)

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December 16, 2004 3:54 pm, GSG_Chapter_4-03.fm

Chapter 3 Designing Primers and Probes for Allelic Discrimination Assays

Automatically Designing Primers and Probes

Primer Express Software 3.0 Getting Started Guide 33

Notes

Figure 12. Probe annotations in Sequence tab

Note: After the software finds primers and probes, the sequence box is locked. To edit

the sequence, click to unlock.

As a general guideline, select the primer/probe sets with a low Penalty score and a low

amplicon length (if the Penalty score and Amplicon Length fields are not displayed,

scroll to the right in the table). However, all primer/probe sets generated using default

parameters meet primer and probe guidelines. For more information regarding Penalty

scores, see Primer Express Software Version 3.0 Online Help.

Saving the

Document

Before proceeding to other designs, be sure to save the Primer/Probe annotations and

results found. Select File > Save As to save the document for future use.

Ordering Primers

and Probes

To order your selected primers and probes, refer to Chapter 4, “Ordering Primers and

Probes.”

IMPORTANT! Before running your samples, Applied Biosystems recommends that you

run control samples to verify the performance of the selected primers and probes.

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AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE Bedienungsanleitung Seite 40